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Italian Movie Download Can Of Worms
A representative movie of odr-10 RNAi treated daf-2(e1370) worm's motility on food. Synchronized daf-2(e1370) eggs were transferred onto NGM plates seeded with odr-10 RNAi bacteria. On Day 2 of adulthood, individual worms were picked from the odr-10 RNAi bacteria plate and transferred to a fresh NGM plate with OP50 bacteria. Immediately after transfer, the movement of the worm was recorded continuously for 10 min. When the worm ran out of the field of view, the plate was relocated to chase the worm as noted by the sudden changes of the field of view in the movie. Note the continuous locomotion of the worm. The movie is 10x faster than real time. At least 10 worms were filmed for the statistical analysis of the locomotive activity. (MOV 19596 kb)
A representative movie of control daf-2(e1370) worm's motility on food. Synchronized eggs (the same population as in supplementary movie 1) were transferred onto NGM plates seeded with OP50 bacteria. On Day 2 of adulthood, individual worms were picked from a NGM plate and transferred to a fresh NGM plate with OP50 bacteria. Immediately after transfer, the movement of the worm was recorded continuously for 10 min. When the worm ran out of the field of view, the plate was relocated to chase the worm as noted by the sudden changes of the field of view in the movie. Note that the worm starts to show a low locomotive activity within 2 min in this case. The movie is 10x faster than real time. At least 10 worms were filmed for the statistical analysis of the locomotive activity. (MOV 33301 kb)
A representative movie of wild type (N2) worm's motility on food. N2 worms were grown on NGM plates seeded with OP50 bacteria. On Day 2 of adulthood, individual worms were picked from a NGM plate and transferred to a fresh NGM plate with OP50 bacteria. Immediately after transfer, the movement of the worm was recorded continuously for 10 min. When the worm ran out of the field of view, the plate was relocated to chase the worm as noted by the sudden changes of the field of view in the movie. Note the continuous locomotion of the worm. The movie is 10x faster than real time. At least 10 worms were filmed for the statistical analysis of the locomotive activity. (MOV 7630 kb)
Caenorhabditis elegans lives for only a few weeks, but during its lifetime it undergoes a physical and behavioural decline that anyone, even someone who has never seen the worm, can recognize as ageing (see electronic supplementary material, online movie). I remember realizing this for the first time in the early 1980s when I was a postdoctoral fellow with Sydney Brenner in Cambridge, UK, and I was cultivating a mutant strain that had very few progeny. Normal C. elegans hermaphrodites produce 300 self-progeny during their first week of life. So a single worm on a culture dish soon disappears into a sea of progeny and cannot be found. I left culture dishes with my almost-infertile mutants in the incubator for several weeks, and then looked at them. With so few progeny, the original animals were still easy to find, and to my surprise, they looked old. This concept, that worms get old, really struck me. I sat there, feeling a little sorry for them, and then wondered whether there were genes that controlled ageing and how one might find them.
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